Ohun elo Iwari Acid Nucleic Acid Pentaplex

【Ipilẹhin】
Arun atẹgun ti o ga julọ (FURD) jẹ ọkan ninu awọn idi pataki julọ ti aisan ati iku ninu awọn ologbo ọdọ. Awọn aami aiṣan ti ile-iwosan aṣoju ti FURD jẹ iba, ounjẹ ti o dinku, ibanujẹ, serous, mucous tabi purulent secretions ninu awọn oju ati iho imu, edema tabi ọgbẹ ninu oropharynx, salivation, ati Ikọaláìdúró lẹẹkọọkan ati sneezing. Awọn ọlọjẹ ti o wọpọ ni feline calicivirus (FCV), feline herpesvirus iru 1 (FHV-I), Mycoplasma (M. felis), Chlamydia felis (C. felis) ati Bordetella bronchiseptica (Bb).

【Ilana ti ilana idanwo】
Feline Respiratory Pathogen Pentaplex Nucleic Acid Detection Apo jẹ idanwo imudara iwọn inu vitro nucleic acid fun acid nucleic ti FHV-1, M. felis, FCV, Bordetella bronchiseptica (Bb) ati C. felis.
Reagenti lyophilized ni awọn orisii alakoko kan pato, awọn iwadii, yiyipada transcriptase, DNA polymerase, dNTPs, surfactant, saarin ati lyoprotectant.
Idanwo yii da lori awọn ilana pataki mẹta: (1) igbaradi ayẹwo adaṣe lati yọkuro lapapọ acid nucleic ti apẹrẹ nipasẹ AIMDX 1800VET; (2) yiyipada transcription ti RNA ibi-afẹde lati ṣe agbekalẹ DNA ibaramu (cDNA); (3) PCR amúṣantóbi ti cDNA ibi-afẹde nipa lilo awọn alakoko ibaramu kan pato, ati wiwa nigbakanna ti awọn iwadii TaqMan cleaved ti o fun laaye wiwa ọja imudara ti awọn ibi-afẹde.